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pre autoclaved lactobacilli mrs broth  (Difco)
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lactobacilli mrs broth bd difco cat 288130  (Difco)
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Heat-killed MI37 stimulates IFN-γ production in CD8 + T cells (A) IFN-γ levels in culture supernatants of splenocytes treated with vehicle, De Man, Rogosa, and Sharpe (MRS) broth, MI37-CFCS, or by KCTC3112-CFCS (KCTC-CFCS) in the presence of 500 ng/mL LPS were measured by ELISA. (B) IFN-γ ELISA of splenocytes treated with viable or heat-killed (HK) bacteria. (C) Representative FACS plots and bar graphs showing the frequency (%) of CD8 + IFN-γ + T cells in splenocytes treated with viable or HK bacteria. (D) Tumor growth in MC38 tumor-bearing mice. (E) IFN-γ levels measured by ELISA in tumor homogenates from MC38 tumor-bearing mice. Data are presented as mean ± SD. Statistical significance was determined using one-way or two-way ANOVA with multiple comparisons. Data are representative of two independent experiments. ∗ p < 0.05 , ∗∗ p < 0.01, ∗∗∗ p < 0.001 .
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mrs broth media  (Beijing Land Bridge Technology Co Ltd)
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Beijing Land Bridge Technology Co Ltd mrs broth media
Heat-killed MI37 stimulates IFN-γ production in CD8 + T cells (A) IFN-γ levels in culture supernatants of splenocytes treated with vehicle, De Man, Rogosa, and Sharpe (MRS) broth, MI37-CFCS, or by KCTC3112-CFCS (KCTC-CFCS) in the presence of 500 ng/mL LPS were measured by ELISA. (B) IFN-γ ELISA of splenocytes treated with viable or heat-killed (HK) bacteria. (C) Representative FACS plots and bar graphs showing the frequency (%) of CD8 + IFN-γ + T cells in splenocytes treated with viable or HK bacteria. (D) Tumor growth in MC38 tumor-bearing mice. (E) IFN-γ levels measured by ELISA in tumor homogenates from MC38 tumor-bearing mice. Data are presented as mean ± SD. Statistical significance was determined using one-way or two-way ANOVA with multiple comparisons. Data are representative of two independent experiments. ∗ p < 0.05 , ∗∗ p < 0.01, ∗∗∗ p < 0.001 .
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Heat-killed MI37 stimulates IFN-γ production in CD8 + T cells (A) IFN-γ levels in culture supernatants of splenocytes treated with vehicle, De Man, Rogosa, and Sharpe (MRS) broth, MI37-CFCS, or by KCTC3112-CFCS (KCTC-CFCS) in the presence of 500 ng/mL LPS were measured by ELISA. (B) IFN-γ ELISA of splenocytes treated with viable or heat-killed (HK) bacteria. (C) Representative FACS plots and bar graphs showing the frequency (%) of CD8 + IFN-γ + T cells in splenocytes treated with viable or HK bacteria. (D) Tumor growth in MC38 tumor-bearing mice. (E) IFN-γ levels measured by ELISA in tumor homogenates from MC38 tumor-bearing mice. Data are presented as mean ± SD. Statistical significance was determined using one-way or two-way ANOVA with multiple comparisons. Data are representative of two independent experiments. ∗ p < 0.05 , ∗∗ p < 0.01, ∗∗∗ p < 0.001 .

Journal: iScience

Article Title: Newly isolated human-derived Lactobacillus fermentum strain stimulates IFN-γ-secreting CD8 + T cells for enhanced anti-cancer immunity

doi: 10.1016/j.isci.2026.115642

Figure Lengend Snippet: Heat-killed MI37 stimulates IFN-γ production in CD8 + T cells (A) IFN-γ levels in culture supernatants of splenocytes treated with vehicle, De Man, Rogosa, and Sharpe (MRS) broth, MI37-CFCS, or by KCTC3112-CFCS (KCTC-CFCS) in the presence of 500 ng/mL LPS were measured by ELISA. (B) IFN-γ ELISA of splenocytes treated with viable or heat-killed (HK) bacteria. (C) Representative FACS plots and bar graphs showing the frequency (%) of CD8 + IFN-γ + T cells in splenocytes treated with viable or HK bacteria. (D) Tumor growth in MC38 tumor-bearing mice. (E) IFN-γ levels measured by ELISA in tumor homogenates from MC38 tumor-bearing mice. Data are presented as mean ± SD. Statistical significance was determined using one-way or two-way ANOVA with multiple comparisons. Data are representative of two independent experiments. ∗ p < 0.05 , ∗∗ p < 0.01, ∗∗∗ p < 0.001 .

Article Snippet: Lactobacilli MRS Broth BD Difco Cat# 288130 , BD Difco , 288130.

Techniques: Enzyme-linked Immunosorbent Assay, Bacteria